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Fluorescent Staining

Fluorescent stainting - click for larger versionThe bacteria status of a root canal can be determined using fluorescent probes. A stain may discriminate between viable cells (green) and killed cells (red). The tissue, here a root slice, is viewed in a confocal microscope to allow thicker samples to be visualized. The left slice shows autofluorescence from dentin, with the dentinal tubules visible. The right slice shows masses of live (green dots) and dead (red dots) bacteria